Suspension for inoculation of the bacteria to be tested is prepared in saline or Mueller–Hinton broth to the turbidity of a 1.5 x 108 cells/ml McFarland standard.
McFarland standards gives the reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within the certain parameter. approximately a culture density should be around 1.5 x 108 cells/ml according to McFarland standards .
McFarland Standards are used to standardize the approximate number of bacteria in a liquid suspension by comparing the turbidity of the test. McFarland Standard contains a chemical solution of barium chloride and sulfuric acid.
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